Comparison of Acetylcholinesterase Assays Run under Conditions Specified by the Standard Ellman Method and Conditions Specified by a Commercial Cholinesterase Reagent Kit
نویسندگان
چکیده
Senior Medical Coordinator, Worker Health and Safety Branch, Department of Pesticide Regulation, 2 Sacramento, California Staff Toxicologist, Worker Health and Safety Branch, Department of Pesticide Regulation, Sacramento, 3 California Associate Toxicologist, Worker Health and Safety Branch, Department of Pesticide Regulation, Sacramento, 4 California Professor of Avian Sciences, Department of Avian Sciences, University of California Davis, Davis, California 5 Comparison of Acetylcholinesterase Assays Run under Conditions Specified by the Standard Ellman Method and Conditions Specified by a Commercial Cholinesterase Reagent Kit July 30, 1998 HS-1752 Jack Henderson Michael O'Malley James Sanborn Marylou Verder-Carlos Barry Wilson
منابع مشابه
Comparative Measurement of Serum Acetyl Cholinesterase Enzyme Using Three Different Methods
Introduction: IAChE (acetylcholinesterase) is one of the plasma’s enzymes that plays a crucial role in nervous signal transduction in synapses and is responsible for the hydrolysis of Ach. Material & Method: We investigated three methods for the determination of AChE in human blood serum, using three different assays. Michel methods are based on the measurement of hydrogen ion produced in pro...
متن کاملComparison of protocols for measuring activities of human blood cholinesterases by the Ellman method.
This paper presents a protocol for routine assays of human blood cholinesterase activities which separates erythrocytes from plasma by centrifugation and measures acetylcholinesterase activity in unwashed erythrocytes and butyrylcholinesterase activity in the plasma. The recommended substrate for both enzymes is 1.0 mM acetylthiocholine. The protocol is compared with other two recommended proto...
متن کاملDecoloration of Disperse blue 56 by Using of UV/H2O2/MWCNT’s
In this study, the oxidation of dye from colored wastewater by H2O2/ MWCNT’s multi walls carbon nano tubes solution assisted with UV radiation was investigated. Disperse Blue 56 DB 56 was used as a model dye. Adsorption behavior of DB56 from aqueous solution onto MWCNT’s was investigated under various conditions such as initial pH value, dye concentration, H2O2 and MWCNT’s dosage. Maximum adsor...
متن کاملThe Comparison of different Procedures for DNA extraction from paraffin-embedded Tissues: A commercial kit and a traditional method based on heating
Abstract Background and objectives: Paraffin-embedded tissues and clinical samples are a valuable resource for molecular genetic studies, but the extraction of high-quality genomic DNA from this tissues is still a problematic issue. In the Present study, the efficiency of two DNA extraction protocols, a commercial kit and a traditional method based on heating and K Proteinase was compared. Mate...
متن کاملThe Effect of Quercetin on Learning and Memory Deficit, Lipid Peroxidation, and Cholinesterase Activeity Following Lipopolysaccharide in the Rat
Background and Objective: Lipolysaccharide (LPS) is a large molecule isolated from bacteria such as the enterobacteriaceae family with a negative effect on memory and learning through disturbing the balance of free radicals and creating oxidative stress conditions. In this study, we evaluated the effect of quercetin on oxidative stress and LPS-induced memory impairment in the rat. Materials a...
متن کامل